Methods of Experiment and Designs of Experiment

Agronomy of crop husbandry and management

Crop protection (biotic and abiotic) and climate change risk management

Tissue culture technology for crop improvement

• Callus, meristem, embryo and anther culture

• Microbial pure culture

Optical, fluorescence and electron microscopy 

Breeding methods of crop improvement

• Marker aided selection (MAS), Hybridization, Backcrossing and Single Seed Descendent (SSD) method

Extraction and assay of biomolecular compounds

• Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA)

• Bio-molecules (sugars, starch, proteins, fatty acids etc.)

• Bioactive compounds (flavonoids, alkaloids, terpenoids, volatiles, hormones etc.)

• Enzymatic and non-enzymatic antioxidants

Biotechnology for crop improvement

• Polymerase Chain Reaction (PCR), Reverse Transcription - Polymerase Chain Reaction (RT-PCR)

• Molecular primer design (SSR and SNP) and Plasmid Vector design

• Recombinant DNA insertion (micropipette, gene gun, electrofusion etc.)

• Agrobacterium mediated gene transfer

• Mutation and Chromosome doubling

• Spectrophotometry and Mass spectrometry

• Microarray, HPLC, ELYSA and gas chromatography

Bioinformatics for novel gene and protein profiling

• Gene databases (NCBI, SMART, Pfam, MEME-suit and GSDS) and Protein database (UNIPROT)

Biological data analysis by R, Statistix10, MEGA, Darwin, PowerMarker, GeneAlex etc.